AP08747PU-N

antibody from Acris Antibodies GmbH

Targeting: SYT1 P65, SVP65, SYT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

AP08747PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP08747PU-N, RRID:AB_1975715

Product name

anti Synaptotagmin pThr202

Antibody type

Polyclonal

Antigen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr202 of Synaptotagmin.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Zebrafish

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Western blot of rat cortex lysate showing specific immunolabeling of the ~60k-62k synaptotagmin phosphorylated at Thr202 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Thr202 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 2. Synaptotagmin pThr202 and PSD95 Double-Label Immunostaining of 14 DIV Cortical Neurons.