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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PPS086 - Provider product page
- Provider
- R&D Systems
- Product name
- Phospho-Synaptotagmin-1 (T202) Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Rat Synaptotagmin phosphorylated at T202
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Simian, Zebrafish
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- For long-term storage,
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Phospho-Synaptotagmin-1 (T202) by Western Blot. Western Blot of rat cortex lysate showing specific immunolabeling of the ~60 - 62 kDa Synaptotagmin phosphorylated at T202 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase; lambda PPase). The blot is identical to the control except that it was incubated in lambda PPase (1200 units for 30 minutes) before being exposed to anti-Synaptotagmin (T202). The immunolabeling is completely eliminated by treatment with lambda PPase.
