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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- NBP1-47280 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-47280, RRID:AB_10010234
- Product name
- Rabbit Polyclonal Jade-1/PHF17 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references A novel non-coding RNA lncRNA-JADE connects DNA damage signalling to histone H4 acetylation.
Wan G, Hu X, Liu Y, Han C, Sood AK, Calin GA, Zhang X, Lu X
The EMBO journal 2013 Oct 30;32(21):2833-47
The EMBO journal 2013 Oct 30;32(21):2833-47
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Jade-1/PHF17 Antibody [NBP1-47280] - Detection of human and mouse Jade-1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Jade-1 antibody NBP1-47280 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Jade-1 was also immunoprecipitated by another rabbit anti-Jade-1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Jade-1/PHF17 Antibody [NBP1-47280] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Jade-1 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Jade-1 was also immunoprecipitated by rabbit anti-Jade-1 antibody BL10256, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
