Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00004925-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004925-M03, RRID:AB_11188548
- Product name
- Mouse Monoclonal Nesfatin-1/Nucleobindin-2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. This product is specific for Human NUCB2 monoclonal antibody (M03), clone 6H4 [Gene ID: 4925].
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nesfatin-1/Nucleobindin-2 Antibody (6H4) [H00004925-M03] - Analysis of NUCB2 expression in HepG2.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Nesfatin-1/Nucleobindin-2 Antibody (6H4) [H00004925-M03] - Detection limit for recombinant GST tagged NUCB2 is 0.3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Nesfatin-1/Nucleobindin-2 Antibody (6H4) [H00004925-M03] - Analysis of monoclonal antibody to NUCB2 on HeLa cell. Antibody concentration 10 ug/ml