Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF5949 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Nesfatin-1/Nucleobindin-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Nesfatin-1/Nucleobindin-2 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P80303
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Nesfatin-1/Nucleobindin-2 by Western Blot. Western blot shows lysates of TK-6 human lymphoblast cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Nesfatin-1/Nucleobindin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5949) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Nesfatin-1/ Nucleobindin-2 between 10 and 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Nesfatin-1/Nucleobindin-2 in Human Mesenchymal Stem Cells. Nesfatin-1/Nucleobindin-2 was detected in immersion fixed human mesenchymal stem cells differentiated into adipocytes using Sheep Anti-Human Nesfatin-1/Nucleobindin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5949) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Golgi. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Nesfatin-1/Nucleobindin-2 in Human Brain. Nesfatin-1/Nucleobindin-2 was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Sheep Anti-Human Nesfatin-1/Nucleobindin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5949) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.