HPA008395

antibody from Atlas Antibodies

Targeting: NUCB2 NEFA

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA008395

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA008395, RRID:AB_1854683

Product name

Anti-NUCB2

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human NUCB2, Gene description: nucleobindin 2, Alternative Gene Names: NEFA, Validated applications: ICC, IHC, WB, Uniprot ID: , Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.2 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

High NUCB2 expression level is associated with metastasis and may promote tumor progression in colorectal cancer

Xie J, Chen L, Chen W
Oncology Letters 2018

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Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy

Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of Proteomics 2012;75(7):2236-2251

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NUCB2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Sample type

Human

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.

Sample type

Human