PA5-34831

antibody from Invitrogen Antibodies

Targeting: ASAH1 AC, ACDase, ASAH, FLJ21558, PHP32

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34831

Provider

Invitrogen Antibodies

Product name

ASAH1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: NT2D1, mouse heart, mouse muscle.

Concentration

1.03 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ASAH1 using 30 µg of NT2D1 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an ASAH1 polyclonal antibody (Product # PA5-34831) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ASAH1 Polyclonal Antibody (Product # PA5-34831). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASAH1 Polyclonal Antibody (Product # PA5-34831) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ASAH1 Polyclonal Antibody detects ASAH1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ASAH1 stained by ASAH1 Polyclonal Antibody (Product # PA5-34831) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using ASAH1 (Product # PA5-34831) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.