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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00000427-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000427-M02, RRID:AB_1111684
- Product name
- ASAH1 monoclonal antibody (M02), clone 1A7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant ASAH1.
- Antigen sequence
PPWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPY
KRWHELMLDKAPMLKVIVNSLKNMINTFVPSGKVM
QVVDEKLPGLLGNFPGPFEEEMKGIAAVTD- Isotype
- IgG
- Antibody clone number
- 1A7
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ASAH1 expression in transfected 293T cell line by ASAH1 monoclonal antibody (M02), clone 1A7.Lane 1: ASAH1 transfected lysate(45 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ASAH1 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to ASAH1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
