Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-52150 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ASAH1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ENSTSYEEAK NLLTKTKILA PAYFILGGNQ SGEGCVITRD RKESLDVYEL DAKQGRWYVV QTNYDRWKHP FFLDDRRTPA KMCLNRTSQE NISFETMYDV LSTKPVLNKL TVYTTLIDVT KGQF
- Concentration
- 0.1 mg/mL
Submitted references α-Galactosidase a Deficiency in Fabry Disease Leads to Extensive Dysregulated Cellular Signaling Pathways in Human Podocytes.
Jehn U, Bayraktar S, Pollmann S, Van Marck V, Weide T, Pavenstädt H, Brand E, Lenders M
International journal of molecular sciences 2021 Oct 20;22(21)
International journal of molecular sciences 2021 Oct 20;22(21)
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ASAH1 in human cell lines SK-MEL-30 and U-251MG using a ASAH1 Polyclonal Antibody (Product # PA5-52150). Corresponding ASAH1 RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-ASAH1 Rabbit Polyclonal Antibody (Product # PA5-52150) and a 40 kDa band corresponding to ASAH1 was observed across cell lines tested. Membrane enriched extracts (30 µg lysate) of HEL 92.1.7 (Lane 1), SK-BR-3 (Lane 2), U-87 MG (Lane 3), A549 (Lane 4), T-47D (Lane 5), Hep G2 (Lane 6), KARPAS 299 (Lane 7) and SH-SY5Y (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). An uncharacterized band (*) at ~38 kDa was observed.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ASAH1 in human heart muscle using an ASAH1 Polyclonal Antibody (Product # PA5-52150) shows strong granular cytoplasmic positivity in cardiomyocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ASAH1 in human kidney using an ASAH1 Polyclonal Antibody (Product # PA5-52150) shows strong granular cytoplasmic positivity in a subset of renal tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ASAH1 in human prostate using an ASAH1 Polyclonal Antibody (Product # PA5-52150) shows strong granular cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ASAH1 in human small intestine using an ASAH1 Polyclonal Antibody (Product # PA5-52150) shows strong granular cytoplasmic positivity in glandular cells.
