- MA5-50633 - Invitrogen Antibodies SLC7A11 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of SLC7A11 in different various lysates. Lane 1: HT-29 cell lysate; Lane 2: A549 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa. Observed band size: 55 kDa. Exposure time: 30 seconds; 10% SDS-PAGE gel. Primary antibody SLC7A11 recombinant monoclonal antibody (Product # MA5-50633) with a dilution of 1:500 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody with a dilution of 1:100,000 was used for 1 hour at room temperature. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SLC7A11 in HCT116 cell lysates. Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa. Observed band size: 55 kDa. Exposure time: 1 minute;12% SDS-PAGE gel. Primary antibody SLC7A11 recombinant monoclonal antibody (Product # MA5-50633) with a dilution of 1:5,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody with a dilution of 1:100,000 was used for 1 hour at room temperature. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SLC7A11 in NIH/3T3 cell lysates. Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa. Observed band size: 55 kDa. Exposure time: 1 minute;8% SDS-PAGE gel. Primary antibody SLC7A11 recombinant monoclonal antibody (Product # MA5-50633) with a dilution of 1:500 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody with a dilution of 1:150,000 was used for 1 hour at room temperature. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of SLC7A11 in HT-29 cells. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were incubated with SLC7A11 recombinant monoclonal antibody (Product # MA5-50633) with a dilution of 1:200 in 2% BSA overnight at 4 ℃. Followed by secondary antibody Goat Anti-Mouse IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry of SLC7A11 in paraffin-embedded human pancreas tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with SLC7A11 recombinant monoclonal antibody (Product # MA5-50633) with a dilution of 1:600 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen, and tissues were counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of SLC7A11 in HT-29 cells. Cells were washed twice with cold PBS and resuspend, then stained with SLC7A11 recombinant monoclonal antibody (Product # MA5-50633) at a dilution of 1 µg/mL (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at 4℃ for 30 minutes, cells were stained with secondary antibody iFluor™ 488 conjugate-Goat anti-Mouse IgG at a dilution of 1:1,000 for 30 minutes at 4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

MA5-50633