PA5-18599

antibody from Invitrogen Antibodies

Targeting: SLC7A11 xCT

Antibody data

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Validation data

Product number: PA5-18599 - Provider product page
Provider: Invitrogen Antibodies
Product name: SLC7A11 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This antibody is predicted to react with canine based on sequence homology.
Reactivity: Human
Host: Goat
Isotype: IgG
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

SLC7A11 protein structure - PA5-18599 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 3 Ferroptosis is enhanced in the kidney of I/R injured rats. Rats renal artery was clamped with non-traumatic clamps for 45 min, followed by restoring of the renal blood flow. Control rats underwent sham surgery (sham group) without ischemia-reperfusion injury (IRI). Blood samples and renal tissues were collected 48 h after the injury. A Serum creatinine and B blood urea nitrogen levels were significantly higher in the I/R group than in the sham group. C Kidney tissue sections were subjected to histological examination by hematoxylin and eosin staining (H&E) and TUNEL assay to evaluate renal tubule injury. D Increased iron level and E ROS level in I/R renal tissues indicates the increase of ferroptosis. F Western blot was used to detect the indicated protein expression. The protein intensity was analyzed by using ImageJ. n = 6. Data are presented as the mean +- s.e.m. of three independent experiments. *** P < 0.001 vs. the sham group.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4 MiR-182-5p and miR-378a-3p negatively correlates with the GPX4 and SLC7A11 expression, respectively. A The representative photograph showing fluorescence in situ hybridization in renal tissues, green represented biotin-labeled probe against miR-182-5p and miR-378a-3p and blue represents DAPI staining of the nucleus. B miR-182-5p and miR-378a-3p expression increased in the I/R renal injury rats. C Western blot was used to detect the GPX4 and SLC7A11 protein expression in the renal tissues. The protein intensity was analyzed by using ImageJ. D Pearson analysis showed the negative correlation of miR-182-5p and miR-378a-3p with GPX4 and SLC7A11 protein expression. n = 6. Data are presented as the mean +- s.e.m. of three independent experiments. *** P < 0.001 vs. the sham group.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 5 MiR-182-5p targets GPX4 and miR-378a-3p targets SLC7A11, respectively, in the renal epithelial cells. A The predicted binding site between 3'UTR of GPX4 mRNA and miR-182-5p, and the predicted binding site between 3'UTR of SLC7A11 mRNA and miR-378a-3p. B HK-2 cells were co-transfected with luciferase constructs containing the GPX4 WT or MUT 3'-UTRs and miR-182-5p mimics or mimics NC, or co-transfected with luciferase constructs containing the SLC7A11 WT or MUT 3'-UTRs and miR-378a-3p mimics or mimics NC. Luciferase activity was measured. C RIP assay followed by qRT-PCR to assay miR-182-5p and miR-378a-3p endogenously associated with GPX4 and SLC7A11, respectively. D miR-182-5p and miR-378a-3p scarcely influence the GPX4 and SLC7A11 mRNA levels. E The GPX4 and SLC7A11 expression levels were downregulated after treated with miR-182-5p and miR-378a-3p mimics and upregulated with the treatment with miRNAs inhibitors in HK-2 and TCMK-1 cells. The protein intensity was analyzed by using ImageJ. n = 6. Data are presented as the mean +- s.e.m. of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001 vs. indicated group.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 6 MiR-182-5p and miR-378a-3p regulates ferroptosis in the renal epithelial cells by targeting GPX4 and SLC7A11, respectively. The HK-2 cells were treated with indicated miRNA mimic (10 pmol) and GPX4 overexpression lentivirus (MOI = 50) with a final concentration of 5 mug/mL polybrene for, or co-treated with the miRNA mimic and lentivirus for 36 h, then the cells were subjected to various detection. nc, control mimic; cv, control lentivirus. A miR-182-5p and miR-378a-3p inhibited the GPX4 and SLC7A11 protein expression which were upregulated by the lentivirus treatment. B Overexpression of GPX4 and SLC7A11 inhibited the reduction of cell viability induced by miRNA mimic. C Overexpression of GPX4 and SLC7A11 inhibited the increase of cell iron level, ROS level ( D ), and mitosox intensity ( E ) induced by miRNA mimic. F Overexpression of GPX4 and SLC7A11 inhibited the miRNA mimic-induced cell death of HK-2 cells. n = 6. Data are presented as the mean +- s.e.m. of three independent experiments. ** P < 0.01, *** P < 0.001 vs. indicated group.