Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- HPA020549 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA020549, RRID:AB_1856834
- Product name
- Anti-SHMT2
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human SHMT2, Gene description: serine hydroxymethyltransferase 2 (mitochondrial), Alternative Gene Names: SHMT, Validated applications: WB, IHC, ICC, Uniprot ID: P34897, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Serine and one-carbon metabolism sustain non-melanoma skin cancer progression
Formate overflow drives toxic folate trapping in MTHFD1 inhibited cancer cells
The mitochondrial protein Sideroflexin 3 (SFXN3) influences neurodegeneration pathwaysin vivo
Mitochondria preserve an autarkic one-carbon cycle to confer growth-independent cancer cell migration and metastasis
One carbon metabolism in human lung cancer
LONP1 and ClpP cooperatively regulate mitochondrial proteostasis for cancer cell survival
Glycine by MR spectroscopy is an imaging biomarker of glioma aggressiveness
Metabolic determinants of cancer cell sensitivity to canonical ferroptosis inducers
PLPHP deficiency: clinical, genetic, biochemical, and mechanistic insights
A PHGDH inhibitor reveals coordination of serine synthesis and one-carbon unit fate
SHMT2 drives glioma cell survival in ischaemia but imposes a dependence on glycine clearance
Cappello A, Zuccotti A, Mancini M, Tosetti G, Fania L, Ricci F, Melino G, Candi E
Cell Death Discovery 2023;9(1)
Cell Death Discovery 2023;9(1)
Formate overflow drives toxic folate trapping in MTHFD1 inhibited cancer cells
Green A, Marttila P, Kiweler N, Chalkiadaki C, Wiita E, Cookson V, Lesur A, Eiden K, Bernardin F, Vallin K, Borhade S, Long M, Ghahe E, Jiménez-Alonso J, Jemth A, Loseva O, Mortusewicz O, Meyers M, Viry E, Johansson A, Hodek O, Homan E, Bonagas N, Ramos L, Sandberg L, Frödin M, Moussay E, Slipicevic A, Letellier E, Paggetti J, Sørensen C, Helleday T, Henriksson M, Meiser J
Nature Metabolism 2023;5(4):642-659
Nature Metabolism 2023;5(4):642-659
The mitochondrial protein Sideroflexin 3 (SFXN3) influences neurodegeneration pathwaysin vivo
Ledahawsky L, Terzenidou M, Edwards R, Kline R, Graham L, Eaton S, van der Hoorn D, Chaytow H, Huang Y, Groen E, Motyl A, Lamont D, Tokatlidis K, Wishart T, Gillingwater T
The FEBS Journal 2022;289(13):3894-3914
The FEBS Journal 2022;289(13):3894-3914
Mitochondria preserve an autarkic one-carbon cycle to confer growth-independent cancer cell migration and metastasis
Kiweler N, Delbrouck C, Pozdeev V, Neises L, Soriano-Baguet L, Eiden K, Xian F, Benzarti M, Haase L, Koncina E, Schmoetten M, Jaeger C, Noman M, Vazquez A, Janji B, Dittmar G, Brenner D, Letellier E, Meiser J
Nature Communications 2022;13(1)
Nature Communications 2022;13(1)
One carbon metabolism in human lung cancer
Yao S, Peng L, Elakad O, Küffer S, Hinterthaner M, Danner B, Hammerstein-Equord A, Ströbel P, Bohnenberger H
Translational Lung Cancer Research 2021;10(6):2523-2538
Translational Lung Cancer Research 2021;10(6):2523-2538
LONP1 and ClpP cooperatively regulate mitochondrial proteostasis for cancer cell survival
Lee Y, Kim H, Nam Y, Shin K, Lee Y, Park D, Rhee H, Seo J, Chae Y
Oncogenesis 2021;10(2)
Oncogenesis 2021;10(2)
Glycine by MR spectroscopy is an imaging biomarker of glioma aggressiveness
Choi C, Maher E, Malloy C, Mickey B, Sherry A, DeBerardinis R, Patel T, Pan E, Madden C, Pinho M, Thomas B, Dimitrov I, Levy M, Baxter J, Askari P, Lewis C, Raisanen J, Ganji S, An Z, Zhang S, Gao A, Hatanpaa K, Daoud E, Tiwari V
Neuro-Oncology 2020;22(7):1018-1029
Neuro-Oncology 2020;22(7):1018-1029
Metabolic determinants of cancer cell sensitivity to canonical ferroptosis inducers
Soula M, Weber R, Zilka O, Alwaseem H, La K, Yen F, Molina H, Garcia-Bermudez J, Pratt D, Birsoy K
Nature Chemical Biology 2020;16(12):1351-1360
Nature Chemical Biology 2020;16(12):1351-1360
PLPHP deficiency: clinical, genetic, biochemical, and mechanistic insights
Johnstone D, Al-Shekaili H, Tarailo-Graovac M, Wolf N, Ivy A, Demarest S, Roussel Y, Ciapaite J, van Roermund C, Kernohan K, Kosuta C, Ban K, Ito Y, McBride S, Al-Thihli K, Abdelrahim R, Koul R, Al Futaisi A, Haaxma C, Olson H, Sigurdardottir L, Arnold G, Gerkes E, Boon M, Heiner-Fokkema M, Noble S, Bosma M, Jans J, Koolen D, Kamsteeg E, Drögemöller B, Ross C, Majewski J, Cho M, Begtrup A, Wasserman W, Bui T, Brimble E, Violante S, Houten S, Wevers R, van Faassen M, Kema I, Lepage N, Lines M, Dyment D, Wanders R, Verhoeven-Duif N, Ekker M, Boycott K, Friedman J, Pena I, van Karnebeek C
Brain 2019;142(3):542-559
Brain 2019;142(3):542-559
A PHGDH inhibitor reveals coordination of serine synthesis and one-carbon unit fate
Pacold M, Brimacombe K, Chan S, Rohde J, Lewis C, Swier L, Possemato R, Chen W, Sullivan L, Fiske B, Cho S, Freinkman E, Birsoy K, Abu-Remaileh M, Shaul Y, Liu C, Zhou M, Koh M, Chung H, Davidson S, Luengo A, Wang A, Xu X, Yasgar A, Liu L, Rai G, Westover K, Vander Heiden M, Shen M, Gray N, Boxer M, Sabatini D
Nature Chemical Biology 2016;12(6):452-458
Nature Chemical Biology 2016;12(6):452-458
SHMT2 drives glioma cell survival in ischaemia but imposes a dependence on glycine clearance
Kim D, Fiske B, Birsoy K, Freinkman E, Kami K, Possemato R, Chudnovsky Y, Pacold M, Chen W, Cantor J, Shelton L, Gui D, Kwon M, Ramkissoon S, Ligon K, Kang S, Snuderl M, Vander Heiden M, Sabatini D
Nature 2015;520(7547):363-367
Nature 2015;520(7547):363-367
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Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
- Sample type
- Human
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human liver and skeletal muscle tissues using HPA020549 antibody. Corresponding SHMT2 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol