11099-1-AP

antibody from Proteintech Group

Targeting: SHMT2 SHMT

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

11099-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#11099-1-AP, RRID:AB_2188452

Product name

SHMT2 antibody

Antibody type

Polyclonal

Description

KD/KO validated SHMT2 antibody (Cat. #11099-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

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Journal of orthopaedic translation 2026 Mar;57:101049

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Native chromatome profiling reveals hundreds of metabolic enzymes in the nucleus across tissues.

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Nature communications 2026 Mar 6;17(1)

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ΔNp63α drives serine synthesis to promote carboplatin resistance in NSCLC.

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Cell death & disease 2026 Feb 17;17(1):227

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Adaptation of white adipocytes to cooler temperatures: impacts on energy metabolism and protein acetylation.

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Single-cell and bulk transcriptome analyses reveal elevated amino acid metabolism promoting tumor-directed immune evasion in colorectal cancer.

Sun T, Chen Y, Chen YX
Frontiers in immunology 2025;16:1575829

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IGF2BP3 redirects glycolytic flux to promote one-carbon metabolism and RNA methylation.

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Conjugated Lithocholic Acid Activates Hepatic TGR5 to Promote Lipotoxicity and MASLD-MASH Transition by Disrupting Carnitine Biosynthesis.

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De Novo Serine Synthesis Is a Metabolic Vulnerability That Can Be Exploited to Overcome Sunitinib Resistance in Advanced Renal Cell Carcinoma.

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Mitochondria-derived nuclear ATP surge protects against confinement-induced proliferation defects.

Ghose R, Pezzano F, Badia R, Kourtis S, Sheraj I, Das S, Gañez Zapater A, Ghose U, Musa-Afaneh S, Espinar L, Coll-Manzano A, Parapatics K, Ivanova S, Sànchez-Fernàndez-de-Landa P, Radivojevikj D, Venturini V, Wieser S, Zorzano A, Müller AC, Ruprecht V, Sdelci S
Nature communications 2025 Jul 30;16(1):6613

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Serum multiomics prediction of prognosis and adverse reactions to concurrent chemoradiotherapy in patients with esophageal cancer.

Wu WZ, Huang HC, Zhu GH, Liao LD, Chen XL, Li ZM, Chu MY, Yu SX, Wang D, Li EM, Xu LY
British journal of cancer 2025 Dec;133(12):1829-1843

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Proteomics and personalized PDX models identify treatment for a progressive malignancy within an actionable timeframe.

Barnabas GD, Bhat TA, Goebeler V, Leclair P, Azzam N, Melong N, Anderson C, Gom A, An S, Ergin EK, Shen Y, Conrrero A, Mungall AJ, Mungall KL, Maxwell CA, Reid GSD, Hirst M, Jones S, Chan JA, Senger DL, Berman JN, Parker SJ, Bush JW, Strahlendorf C, Deyell RJ, Lim CJ, Lange PF
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Interaction Between the PERK/ATF4 Branch of the Endoplasmic Reticulum Stress and Mitochondrial One-Carbon Metabolism Regulates Neuronal Survival After Intracerebral Hemorrhage.

Liu Y, Cui F, Xu A, Wang B, Ma Y, Zhang Q, Sun Q, Zheng Y, Xue Y, Sun Y, Bian L
International journal of biological sciences 2024;20(11):4277-4296

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RNAi screens identify HES4 as a regulator of redox balance supporting pyrimidine synthesis and tumor growth.

He J, Wang A, Zhao Q, Zou Y, Zhang Z, Sha N, Hou G, Zhou B, Yang Y, Chen T, Zhao Y, Jiang Y
Nature structural & molecular biology 2024 Sep;31(9):1413-1425

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Multiomics analysis reveals serine catabolism as a potential therapeutic target for MELAS.

Liufu T, Zhao X, Yu M, Xie Z, Meng L, Lv H, Zhang W, Yuan Y, Xing G, Deng J, Wang Z
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Jun 30;38(12):e23742

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CYP3A5 unexpectedly regulates glucose metabolism through the AKT-TXNIP-GLUT1 axis in pancreatic cancer.

Shao M, Pan Q, Tan H, Wu J, Lee HW, Huber AD, Wright WC, Cho JH, Yu J, Peng J, Chen T
Genes & diseases 2024 Jul;11(4):101079

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Comparative Analysis of Two NNMT Bisubstrate Inhibitors through Chemoproteomic Studies: Uncovering the Role of Unconventional SAM Analogue Moiety for Improved Selectivity.

Meng Y, Iyamu ID, Ahmed NAM, Huang R
ACS chemical biology 2024 Jan 19;19(1):89-100

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Redox homeostasis of one-carbon metabolism-dependent reprogramming is critical for RCC progression under exogenous serine/glycine-deprived conditions.

Wang H, Fan M, Liu S, Qu M, Hou X, Hou J, Xu Y, Shang X, Liu C, He M, Gao J, Chen J, Li X
BMC cancer 2024 Dec 18;24(1):1515

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Construction and validation of a folate metabolism-related gene signature for predicting prognosis in HNSCC.

Wang L, He Y, Bai Y, Zhang S, Pang B, Chen A, Wu X
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Differential Inhibition of Anaplerotic Pyruvate Carboxylation and Glutaminolysis-Fueled Anabolism Underlies Distinct Toxicity of Selenium Agents in Human Lung Cancer.

Fan TW, Winnike J, Al-Attar A, Belshoff AC, Lorkiewicz PK, Tan JL, Wu M, Higashi RM, Lane AN
Metabolites 2023 Jun 21;13(7)

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Multi-omics reveals that green pea (Pisum sativum L.) hull supplementation ameliorates non-alcoholic fatty liver disease via the SHMT2/glycine/mTOR/PPAR-γ signaling pathway.

Guo F, Xiong H, Tsao R, Wen X, Liu J, Chen D, Jiang L, Sun Y
Food & function 2023 Jul 31;14(15):7195-7208

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Serine hydroxymethyltransferase 2 knockdown induces apoptosis in ccRCC by causing lysosomal membrane permeabilization via metabolic reprogramming.

Liu Z, Fan M, Hou J, Pan S, Xu Y, Zhang H, Liu C, Hao X, Li X, Wang H
Cell death & disease 2023 Feb 20;14(2):144

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Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein.

Höllerhage M, Stepath M, Kohl M, Pfeiffer K, Chua OWH, Duan L, Hopfner F, Eisenacher M, Marcus K, Höglinger GU
Frontiers in neurology 2022;13:787059

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SHMT2 promotes the tumorigenesis of renal cell carcinoma by regulating the m6A modification of PPAT.

Huo FC, Xie M, Zhu ZM, Zheng JN, Pei DS
Genomics 2022 Jul;114(4):110424

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Overexpression of SHMT2 Predicts a Poor Prognosis and Promotes Tumor Cell Growth in Bladder Cancer.

Zhang P, Yang Q
Frontiers in genetics 2021;12:682856

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Derangement of hepatic polyamine, folate, and methionine cycle metabolism in cystathionine beta-synthase-deficient homocystinuria in the presence and absence of treatment: Possible implications for pathogenesis.

Maclean KN, Jiang H, Phinney WN, Mclagan BM, Roede JR, Stabler SP
Molecular genetics and metabolism 2021 Feb;132(2):128-138

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Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.

Cui L, Zhao X, Jin Z, Wang H, Yang SF, Hu S
Journal of pineal research 2021 Dec;71(4):e12767

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Generation of SHMT2 knockout human embryonic stem cell line (WAe009-A-67) using CRISPR/Cas9 technique.

Li J, Zhang B, Gan M, Li Y, He L, Yue W, Qiao H, Pei X, Li Y
Stem cell research 2021 Dec;57:102581

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TGF-β Promotes Metabolic Reprogramming in Lung Fibroblasts via mTORC1-dependent ATF4 Activation.

O'Leary EM, Tian Y, Nigdelioglu R, Witt LJ, Cetin-Atalay R, Meliton AY, Woods PS, Kimmig LM, Sun KA, Gökalp GA, Mutlu GM, Hamanaka RB
American journal of respiratory cell and molecular biology 2020 Nov;63(5):601-612

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Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death.

Balsa E, Perry EA, Bennett CF, Jedrychowski M, Gygi SP, Doench JG, Puigserver P
Nature communications 2020 Jun 1;11(1):2714

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De novo synthesis of serine and glycine fuels purine nucleotide biosynthesis in human lung cancer tissues.

Fan TWM, Bruntz RC, Yang Y, Song H, Chernyavskaya Y, Deng P, Zhang Y, Shah PP, Beverly LJ, Qi Z, Mahan AL, Higashi RM, Dang CV, Lane AN
The Journal of biological chemistry 2019 Sep 6;294(36):13464-13477

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Inhibition of Phosphoglycerate Dehydrogenase Attenuates Bleomycin-induced Pulmonary Fibrosis.

Hamanaka RB, Nigdelioglu R, Meliton AY, Tian Y, Witt LJ, O'Leary E, Sun KA, Woods PS, Wu D, Ansbro B, Ard S, Rohde JM, Dulin NO, Guzy RD, Mutlu GM
American journal of respiratory cell and molecular biology 2018 May;58(5):585-593

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SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.

Xu M, Moresco JJ, Chang M, Mukim A, Smith D, Diedrich JK, Yates JR 3rd, Jones KA
PLoS pathogens 2018 May;14(5):e1007071

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Vascular endothelial growth factor signaling requires glycine to promote angiogenesis.

Guo D, Murdoch CE, Xu H, Shi H, Duan DD, Ahmed A, Gu Y
Scientific reports 2017 Nov 7;7(1):14749

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Transforming Growth Factor (TGF)-β Promotes de Novo Serine Synthesis for Collagen Production.

Nigdelioglu R, Hamanaka RB, Meliton AY, O'Leary E, Witt LJ, Cho T, Sun K, Bonham C, Wu D, Woods PS, Husain AN, Wolfgeher D, Dulin NO, Chandel NS, Mutlu GM
The Journal of biological chemistry 2016 Dec 30;291(53):27239-27251

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'Rod and ring' formation from IMP dehydrogenase is regulated through the one-carbon metabolic pathway.

Calise SJ, Purich DL, Nguyen T, Saleem DA, Krueger C, Yin JD, Chan EK
Journal of cell science 2016 Aug 1;129(15):3042-52

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cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Sun L, Song L, Wan Q, Wu G, Li X, Wang Y, Wang J, Liu Z, Zhong X, He X, Shen S, Pan X, Li A, Wang Y, Gao P, Tang H, Zhang H
Cell research 2015 Apr;25(4):429-44

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11099-1-AP(SHMT2 antibody) at dilution of 1:800

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The SHMT2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human SHMT2. This antibody recognizes human, mouse, rat antigen. The SHMT2 antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

11099-1-AP.pdf

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