Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF2730 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse RELM beta Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse RELM beta in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant rat RELM gamma is observed and 10% cross-reactivity with recombinant mouse (rm) Resistin and rmRELM alpha is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q99P86
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Lactobacillus salivarius reverse antibiotic-induced lung defense impairment in a ventilator model.
Tsay TB, Yang MC, Chang WH, Chen PH, Chen LW
Journal of translational medicine 2018 Aug 13;16(1):225
Journal of translational medicine 2018 Aug 13;16(1):225
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse RELM beta by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse RELM beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2730) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RELM beta at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.