Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF1278 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal TREM1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human TREM-1 in ELISAs and Western blots. In ELISAs, less than 0.2% cross-reactivity with recombinant mouse (rm) TREM-1 and rmTREM-2b is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Metalloproteinases shed TREM-1 ectodomain from lipopolysaccharide-stimulated human monocytes.
Metalloproteinases shed TREM-1 ectodomain from lipopolysaccharide-stimulated human monocytes.
Gómez-Piña V, Soares-Schanoski A, Rodríguez-Rojas A, Del Fresno C, García F, Vallejo-Cremades MT, Fernández-Ruiz I, Arnalich F, Fuentes-Prior P, López-Collazo E
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):4065-73
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):4065-73
Metalloproteinases shed TREM-1 ectodomain from lipopolysaccharide-stimulated human monocytes.
Gómez-Piña V, Soares-Schanoski A, Rodríguez-Rojas A, Del Fresno C, García F, Vallejo-Cremades MT, Fernández-Ruiz I, Arnalich F, Fuentes-Prior P, López-Collazo E
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):4065-73
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):4065-73
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human TREM-1 by Western Blot. Western blot shows lysates of human granulocytes. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TREM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1278) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TREM-1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.