Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 50-3549-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD354 (TREM-1) Monoclonal Antibody (Lndnb3r), eFluor 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The Lndnb3r monoclonal antibody recognizes human CD354 (also known as TREM-1), a 30-kDa glycosylated member of the Ig superfamily. CD354 is expressed on the cell surface of myeloid cells such as neutrophils, monocytes, dendritic cells, some macrophages, platelets, and megakaryocytes. Outside the immune system, CD354 is expressed on hepatocytes, osteoclasts, and microglia. CD354 also exists as a soluble receptor (sTREM-1) that arises from proteolytic cleavage of the membrane-bound receptor by matrix metalloproteases. CD354 plays a critical role in the innate immune response to bacterial and fungal infection by triggering the release of pro-inflammatory chemokines and cytokines. The cell surface receptor associates constitutively with the ITAM-containing adaptor protein DAP12 to activate the MAPK and NFkB signaling pathways. Finally, levels of the soluble receptor are increased in several inflammatory diseases, including sepsis, asthma, and inflammatory bowel disease, making this receptor a valuable diagnostic biomarker. Applications Reported: This Lndnb3r antibody has been reported for use in flow cytometric analysis. Applications Tested: This Lndnb3r antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- Lndnb3r
- Vial size
- 100 Tests
- Concentration
- 5 µl/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references TREM-1: intracellular signaling pathways and interaction with pattern recognition receptors.
TREM-1--expressing intestinal macrophages crucially amplify chronic inflammation in experimental colitis and inflammatory bowel diseases.
Soluble triggering receptor expressed on myeloid cells 1 as an anti-inflammatory mediator in sepsis.
TREM-1, MDL-1, and DAP12 expression is associated with a mature stage of myeloid development.
Arts RJ, Joosten LA, van der Meer JW, Netea MG
Journal of leukocyte biology 2013 Feb;93(2):209-15
Journal of leukocyte biology 2013 Feb;93(2):209-15
TREM-1--expressing intestinal macrophages crucially amplify chronic inflammation in experimental colitis and inflammatory bowel diseases.
Schenk M, Bouchon A, Seibold F, Mueller C
The Journal of clinical investigation 2007 Oct;117(10):3097-106
The Journal of clinical investigation 2007 Oct;117(10):3097-106
Soluble triggering receptor expressed on myeloid cells 1 as an anti-inflammatory mediator in sepsis.
Giamarellos-Bourboulis EJ, Zakynthinos S, Baziaka F, Papadomichelakis E, Virtzili S, Koutoukas P, Armaganidis A, Giamarellou H, Roussos C
Intensive care medicine 2006 Feb;32(2):237-243
Intensive care medicine 2006 Feb;32(2):237-243
TREM-1, MDL-1, and DAP12 expression is associated with a mature stage of myeloid development.
Gingras MC, Lapillonne H, Margolin JF
Molecular immunology 2002 Mar;38(11):817-24
Molecular immunology 2002 Mar;38(11):817-24
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD354 (TREM-1) eFluor® 660. Cells in the lymphocyte (blue), monocyte (orange), and granulocyte (purple) gates were used for analysis.