50-3549-42

antibody from Invitrogen Antibodies
Targeting: TREM1 CD354, TREM-1
Provider product page for 50-3549-42

Antibody data

Product number
50-3549-42 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-CD354 (TREM-1) Monoclonal Antibody (Lndnb3r), eFluor 660, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The Lndnb3r monoclonal antibody recognizes human CD354 (also known as TREM-1), a 30-kDa glycosylated member of the Ig superfamily. CD354 is expressed on the cell surface of myeloid cells such as neutrophils, monocytes, dendritic cells, some macrophages, platelets, and megakaryocytes. Outside the immune system, CD354 is expressed on hepatocytes, osteoclasts, and microglia. CD354 also exists as a soluble receptor (sTREM-1) that arises from proteolytic cleavage of the membrane-bound receptor by matrix metalloproteases. CD354 plays a critical role in the innate immune response to bacterial and fungal infection by triggering the release of pro-inflammatory chemokines and cytokines. The cell surface receptor associates constitutively with the ITAM-containing adaptor protein DAP12 to activate the MAPK and NFkB signaling pathways. Finally, levels of the soluble receptor are increased in several inflammatory diseases, including sepsis, asthma, and inflammatory bowel disease, making this receptor a valuable diagnostic biomarker. Applications Reported: This Lndnb3r antibody has been reported for use in flow cytometric analysis. Applications Tested: This Lndnb3r antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
Lndnb3r
Vial size
100 Tests
Concentration
5 µl/Test
Storage
4° C, store in dark, DO NOT FREEZE!
TREM1 protein structure - 50-3549-42 shown in red.