- MA1-12293 - Invitrogen Antibodies CDC25A antibody

Submitted references β-HPV 8E6 Attenuates ATM and ATR Signaling in Response to UV Damage.

Snow JA, Murthy V, Dacus D, Hu C, Wallace NA
Pathogens (Basel, Switzerland) 2019 Nov 26;8(4)

Mangrove dolabrane-type of diterpenes tagalsins suppresses tumor growth via ROS-mediated apoptosis and ATM/ATR-Chk1/Chk2-regulated cell cycle arrest.

Neumann J, Yang Y, Köhler R, Giaisi M, Witzens-Harig M, Liu D, Krammer PH, Lin W, Li-Weber M
International journal of cancer 2015 Dec 1;137(11):2739-48

Overexpression of CDC25B, CDC25C and phospho-CDC25C (Ser216) in vulvar squamous cell carcinomas are associated with malignant features and aggressive cancer phenotypes.

Wang Z, Trope CG, Flørenes VA, Suo Z, Nesland JM, Holm R
BMC cancer 2010 May 25;10:233

Dual regulation of Cdc25A by Chk1 and p53-ATF3 in DNA replication checkpoint control.

Demidova AR, Aau MY, Zhuang L, Yu Q
The Journal of biological chemistry 2009 Feb 13;284(7):4132-9

The F box protein Fbx6 regulates Chk1 stability and cellular sensitivity to replication stress.

Zhang YW, Brognard J, Coughlin C, You Z, Dolled-Filhart M, Aslanian A, Manning G, Abraham RT, Hunter T
Molecular cell 2009 Aug 28;35(4):442-53

Extensive alteration in the expression profiles of TGFB pathway signaling components and TP53 is observed along the gastric dysplasia-carcinoma sequence.

Kim SH, Lee SH, Choi YL, Wang LH, Park CK, Shin YK
Histology and histopathology 2008 Dec;23(12):1439-52

The let-7 microRNA represses cell proliferation pathways in human cells.

Johnson CD, Esquela-Kerscher A, Stefani G, Byrom M, Kelnar K, Ovcharenko D, Wilson M, Wang X, Shelton J, Shingara J, Chin L, Brown D, Slack FJ
Cancer research 2007 Aug 15;67(16):7713-22

How adeno-associated virus Rep78 protein arrests cells completely in S phase.

Berthet C, Raj K, Saudan P, Beard P
Proceedings of the National Academy of Sciences of the United States of America 2005 Sep 20;102(38):13634-9

Estrogen receptor beta inhibits 17beta-estradiol-stimulated proliferation of the breast cancer cell line T47D.

Ström A, Hartman J, Foster JS, Kietz S, Wimalasena J, Gustafsson JA
Proceedings of the National Academy of Sciences of the United States of America 2004 Feb 10;101(6):1566-71

Chk1 mediates S and G2 arrests through Cdc25A degradation in response to DNA-damaging agents.

Xiao Z, Chen Z, Gunasekera AH, Sowin TJ, Rosenberg SH, Fesik S, Zhang H
The Journal of biological chemistry 2003 Jun 13;278(24):21767-73

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Expression of CDC25 protein in vulvar squmous epithelium . Immunohistochemical staining of CDC25A (a), CDC25B (b), CDC25C (c) and phospho-CDC25C (Ser216) (d) in normal vulvar epithelium. CDC25A (e), CDC25B (f), CDC25C (g) and phospho-CDC25C (Ser216) (h) staining in vulvar carcinomas.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Expression of CDC25 protein in vulvar cancer cell line . Immunohistochemical staining of CDC25A (a), CDC25B (b), CDC25C (c) and phospho-CDC25C (Ser216) (d) in CAL-39 cell line. Western blot analysis of CDC25s cellular localization in CAL-39 cells (e). Cell fractions of cytoplasm (lane 1) and nucleus (lane 2) were immunoblotted with Tubulin and Lamin B antibodies to verify the purity of subcellular fraction extraction, then with CDC25A, CDC25B, CDC25C and phospho-CDC25C (Ser216) antibodies, respectively. Cyt: Cytoplasmic fraction; Nuc: Nuclear fraction.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 beta-HPV 8E6 attenuates CHK1 phosphorylation. ( A ) Representative immunoblots of untreated hTERT HFKs with vector control (LXSN) and beta-HPV 8E6 cell lines. Nucleolin was used as a loading control. ( B ) mRNA expression level of CHEK1 in vector control (LXSN) and beta-HPV 8E6 expressing primary HFKs as measured by RT-qPCR and normalized towards the expression level of beta-actin. Data shown in figures are the means of +-SE of three independent experiments. ( C ) Representative immunoblots of hTERT HFKs with vector control (LXSN) and beta-HPV 8E6 harvested 0-6 h post 5mJ/cm 2 UVR. Nucleolin was used as a loading control. ( D ) Representative immunoblots of primary HFKs with vector control (LXSN) and beta-HPV 8E6 harvested 0-8 h post 5 mJ/cm 2 UVR. Nucleolin was used as a loading control. ( E ) Representative immunoblots of hTERT HFKs with vector control (LXSN) and beta-HPV 8E6 harvested 0-8 h post 5 mJ/cm 2 UVR. Nucleolin was used as a loading control. ( A, C - E ) The numbers above bands represent quantification by densitometry. This is shown relative to untreated cells within the same cell line and normalized to the loading control. ( F ) Cell cycle analysis of hTERT HFKs with LXSN vector control and beta-HPV 8E6 1 h post 5 mJ/cm 2 UVR.

MA1-12293

Antibody data