AP3045a

antibody from Abgent

Targeting: CDC25A

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP3045a

Provider

Abgent

Proper citation

Abgent Cat#AP3045a, RRID:AB_637587

Product name

Phospho-CDC25A(S124) Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

Submitted references

CHEK2 genomic and proteomic analyses reveal genetic inactivation or endogenous activation across the 60 cell lines of the US National Cancer Institute.

Zoppoli G, Solier S, Reinhold WC, Liu H, Connelly JW Jr, Monks A, Shoemaker RH, Abaan OD, Davis SR, Meltzer PS, Doroshow JH, Pommier Y
Oncogene 2012 Jan 26;31(4):403-18

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Death receptor-induced activation of the Chk2- and histone H2AX-associated DNA damage response pathways.

Solier S, Sordet O, Kohn KW, Pommier Y
Molecular and cellular biology 2009 Jan;29(1):68-82

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

The anti-phospho CDC25A S124 Pab (Cat. #AP3045a) is used in Western blot to detect CDC25A in mouse liver tissue lysate

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."

Primary Ab dilution

1:50~100