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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP3046a - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP3046a, RRID:AB_637588
- Product name
- Phospho-CDC25A(S177) Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Methanol extract of the ethnopharmaceutical remedy Smilax spinosa exhibits anti-neoplastic activity.
Hsp90 stabilizes Cdc25A and counteracts heat shock-mediated Cdc25A degradation and cell-cycle attenuation in pancreatic carcinoma cells.
An apolar extract of Critonia morifolia inhibits c-Myc, cyclin D1, Cdc25A, Cdc25B, Cdc25C and Akt and induces apoptosis.
A novel N-hydroxy-N'-aminoguanidine derivative inhibits ribonucleotide reductase activity: Effects in human HL-60 promyelocytic leukemia cells and synergism with arabinofuranosylcytosine (Ara-C).
Berberine and a Berberis lycium extract inactivate Cdc25A and induce alpha-tubulin acetylation that correlate with HL-60 cell cycle inhibition and apoptosis.
Pro- and anticarcinogenic mechanisms of piceatannol are activated dose dependently in MCF-7 breast cancer cells.
Short 42 degrees C heat shock induces phosphorylation and degradation of Cdc25A which depends on p38MAPK, Chk2 and 14.3.3.
Carboxyl-terminal peptides as probes for Escherichia coli ribonucleotide reductase subunit interaction: kinetic analysis of inhibition studies.
Seelinger M, Popescu R, Giessrigl B, Jarukamjorn K, Unger C, Wallnöfer B, Fritzer-Szekeres M, Szekeres T, Diaz R, Jäger W, Frisch R, Kopp B, Krupitza G
International journal of oncology 2012 Sep;41(3):1164-72
International journal of oncology 2012 Sep;41(3):1164-72
Hsp90 stabilizes Cdc25A and counteracts heat shock-mediated Cdc25A degradation and cell-cycle attenuation in pancreatic carcinoma cells.
Giessrigl B, Krieger S, Rosner M, Huttary N, Saiko P, Alami M, Messaoudi S, Peyrat JF, Maciuk A, Gollinger M, Kopf S, Kazlauskas E, Mazal P, Szekeres T, Hengstschläger M, Matulis D, Jäger W, Krupitza G
Human molecular genetics 2012 Nov 1;21(21):4615-27
Human molecular genetics 2012 Nov 1;21(21):4615-27
An apolar extract of Critonia morifolia inhibits c-Myc, cyclin D1, Cdc25A, Cdc25B, Cdc25C and Akt and induces apoptosis.
Unger C, Popescu R, Giessrigl B, Rarova L, Herbacek I, Seelinger M, Diaz R, Wallnöfer B, Fritzer-Szekeres M, Szekeres T, Frisch R, Doležal K, Strnad M, De Martin R, Grusch M, Kopp B, Krupitza G
International journal of oncology 2012 Jun;40(6):2131-9
International journal of oncology 2012 Jun;40(6):2131-9
A novel N-hydroxy-N'-aminoguanidine derivative inhibits ribonucleotide reductase activity: Effects in human HL-60 promyelocytic leukemia cells and synergism with arabinofuranosylcytosine (Ara-C).
Saiko P, Graser G, Giessrigl B, Lackner A, Grusch M, Krupitza G, Basu A, Sinha BN, Jayaprakash V, Jaeger W, Fritzer-Szekeres M, Szekeres T
Biochemical pharmacology 2011 Jan 1;81(1):50-9
Biochemical pharmacology 2011 Jan 1;81(1):50-9
Berberine and a Berberis lycium extract inactivate Cdc25A and induce alpha-tubulin acetylation that correlate with HL-60 cell cycle inhibition and apoptosis.
Khan M, Giessrigl B, Vonach C, Madlener S, Prinz S, Herbaceck I, Hölzl C, Bauer S, Viola K, Mikulits W, Quereshi RA, Knasmüller S, Grusch M, Kopp B, Krupitza G
Mutation research 2010 Jan 5;683(1-2):123-30
Mutation research 2010 Jan 5;683(1-2):123-30
Pro- and anticarcinogenic mechanisms of piceatannol are activated dose dependently in MCF-7 breast cancer cells.
Vo NT, Madlener S, Bago-Horvath Z, Herbacek I, Stark N, Gridling M, Probst P, Giessrigl B, Bauer S, Vonach C, Saiko P, Grusch M, Szekeres T, Fritzer-Szekeres M, Jäger W, Krupitza G, Soleiman A
Carcinogenesis 2010 Dec;31(12):2074-81
Carcinogenesis 2010 Dec;31(12):2074-81
Short 42 degrees C heat shock induces phosphorylation and degradation of Cdc25A which depends on p38MAPK, Chk2 and 14.3.3.
Madlener S, Rosner M, Krieger S, Giessrigl B, Gridling M, Vo TP, Leisser C, Lackner A, Raab I, Grusch M, Hengstschläger M, Dolznig H, Krupitza G
Human molecular genetics 2009 Jun 1;18(11):1990-2000
Human molecular genetics 2009 Jun 1;18(11):1990-2000
Carboxyl-terminal peptides as probes for Escherichia coli ribonucleotide reductase subunit interaction: kinetic analysis of inhibition studies.
Climent I, Sjöberg BM, Huang CY
Biochemistry 1991 May 28;30(21):5164-71
Biochemistry 1991 May 28;30(21):5164-71
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- The anti-Phospho-CDC25A-S177 Pab (Cat. #AP3046a) is used in Western blot to detect Phospho-CDC25A-S177 in mouse kidney tissue lysate
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
