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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB0430 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0430, RRID:AB_982425
- Product name
- CDC25A (phospho T507) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC25A.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Cdc25A and cdc25B expression in malignant lymphoma of the thyroid: correlation with histological subtypes and cell proliferation.
Activation of the Raf-1/MEK/Erk kinase pathway by a novel Cdc25 inhibitor in human prostate cancer cells.
Regulation of human Cdc25A stability by Serine 75 phosphorylation is not sufficient to activate a S phase checkpoint.
Ito Y, Yoshida H, Matsuzuka F, Matsuura N, Nakamura Y, Nakamine H, Kakudo K, Kuma K, Miyauchi A
International journal of molecular medicine 2004 Mar;13(3):431-5
International journal of molecular medicine 2004 Mar;13(3):431-5
Activation of the Raf-1/MEK/Erk kinase pathway by a novel Cdc25 inhibitor in human prostate cancer cells.
Nemoto K, Vogt A, Oguri T, Lazo JS
The Prostate 2004 Jan 1;58(1):95-102
The Prostate 2004 Jan 1;58(1):95-102
Regulation of human Cdc25A stability by Serine 75 phosphorylation is not sufficient to activate a S phase checkpoint.
Goloudina A, Yamaguchi H, Chervyakova DB, Appella E, Fornace AJ Jr, Bulavin DV
Cell cycle (Georgetown, Tex.) 2003 Sep-Oct;2(5):473-8
Cell cycle (Georgetown, Tex.) 2003 Sep-Oct;2(5):473-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CDC25A (phospho T506) polyclonal antibody (Cat # PAB0430). Western blot analysis of CDC25A expression in human colon.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The CDC25A (phospho T506) polyclonal antibody (Cat # PAB0430) is used in Western blot to detect Phospho-CDC25A-T506 in cells transfected with wild type or mutant T506A of CDC25A. Data courtesy of Dr. Tiebang Kang of Washington University, St. Louis, MO.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with CDC25A (phospho T506) polyclonal antibody (Cat # PAB0430) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
