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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Product number
- ABIN358097 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer76) antibody
- Antibody type
- Polyclonal
- Description
- Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- pSer76
- Vial size
- 0.4 mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Cdc25A and cdc25B expression in malignant lymphoma of the thyroid: correlation with histological subtypes and cell proliferation.
Activation of the Raf-1/MEK/Erk kinase pathway by a novel Cdc25 inhibitor in human prostate cancer cells.
Regulation of human Cdc25A stability by Serine 75 phosphorylation is not sufficient to activate a S phase checkpoint.
DNA damage during the spindle-assembly checkpoint degrades CDC25A, inhibits cyclin-CDC2 complexes, and reverses cells to interphase.
Chk1 kinase negatively regulates mitotic function of Cdc25A phosphatase through 14-3-3 binding.
Ito Y, Yoshida H, Matsuzuka F, Matsuura N, Nakamura Y, Nakamine H, Kakudo K, Kuma K, Miyauchi A
International journal of molecular medicine 2004 Mar;13(3):431-5
International journal of molecular medicine 2004 Mar;13(3):431-5
Activation of the Raf-1/MEK/Erk kinase pathway by a novel Cdc25 inhibitor in human prostate cancer cells.
Nemoto K, Vogt A, Oguri T, Lazo JS
The Prostate 2004 Jan 1;58(1):95-102
The Prostate 2004 Jan 1;58(1):95-102
Regulation of human Cdc25A stability by Serine 75 phosphorylation is not sufficient to activate a S phase checkpoint.
Goloudina A, Yamaguchi H, Chervyakova DB, Appella E, Fornace AJ Jr, Bulavin DV
Cell cycle (Georgetown, Tex.) 2003 Sep-Oct;2(5):473-8
Cell cycle (Georgetown, Tex.) 2003 Sep-Oct;2(5):473-8
DNA damage during the spindle-assembly checkpoint degrades CDC25A, inhibits cyclin-CDC2 complexes, and reverses cells to interphase.
Chow JP, Siu WY, Fung TK, Chan WM, Lau A, Arooz T, Ng CP, Yamashita K, Poon RY
Molecular biology of the cell 2003 Oct;14(10):3989-4002
Molecular biology of the cell 2003 Oct;14(10):3989-4002
Chk1 kinase negatively regulates mitotic function of Cdc25A phosphatase through 14-3-3 binding.
Chen MS, Ryan CE, Piwnica-Worms H
Molecular and cellular biology 2003 Nov;23(21):7488-97
Molecular and cellular biology 2003 Nov;23(21):7488-97
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