Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 10-7554 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-CDC25A Antibody
- Antibody type
- Monoclonal
- Description
- CDC25A is a dual specificity (tyr/thr) phosphatase and a putative upregulated c-Myc target gene. It is a regulator of the cell cycle required for progression from G1 to S phase. CDC25A is a member of the CDC25 family of phosphatases. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. Overexpression of CDC25A bypassed the mechanism of cell cycle arrest, leading to enhanced DNA damage and decreased cell survival. Specific degradation of CDC25A is identified as part of the DNA damage checkpoint mechanism and suggested how CDC25A overexpression in human cancers might contribute to tumorigenesis.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant CDC25A
protein (amino acids 190-484) was
used as the immunogen for this anti
body.- Isotype
- IgG
- Antibody clone number
- ABM4A46
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
No comments: Submit comment
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of CDC25A. Anti-CDC25A antibody (Clone: ABM4A46) was tested at 0.5 µg/ml and 2 µg/ml on (1) recombinant protein and (2) human heart lysates respectively.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CDC25A in human tonsil tissue using CDC25A antibody (Clone: ABM4A46) at 5 µg/ml.
- Protocol
- Protocol