LS-C800195

antibody from LSBio

Targeting: CDC25A

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C800195

Provider

LSBio

Product name

CDC25A Antibody (phospho-Ser75) LS-C800195

Antibody type

Polyclonal

Description

Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis on HeLa cell lysates using Phospho-CDC25A(Ser75) antibody. The lane on the left is treated with the antigen-specific peptide.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis on HeLa cell lysates using Phospho-CDC25A(Ser75) antibody. The lane on the left is treated with the antigen-specific peptide.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

1:100 staining mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.