NB100-213

antibody from Novus Biologicals

Targeting: CDC25A

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NB100-213

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-213, RRID:AB_10003027

Product name

Rabbit Polyclonal CDC25A Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

The role of Med19 in the proliferation and tumorigenesis of human hepatocellular carcinoma cells.

Zou SW, Ai KX, Wang ZG, Yuan Z, Yan J, Zheng Q
Acta pharmacologica Sinica 2011 Mar;32(3):354-60

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CDC25A Antibody [NB100-213] - Detection of Human CDC25a by Western Blot. Samples: Nuclear extract (50 ug) from HeLa and 293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody NB100-213 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CDC25A Antibody [NB100-213] - Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-CDC25a antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CDC25A Antibody [NB100-213] - Detection of human CDC25a by western blot. Samples: Nuclear extract (50 µg) from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody NB100-213 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.