PA5-12564

antibody from Invitrogen Antibodies

Targeting: CDC25A

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-12564

Provider

Invitrogen Antibodies

Product name

Phospho-Cdc25A (Thr507) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, mouse and rat based on sequence homology.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

β-HPV 8E6 Attenuates ATM and ATR Signaling in Response to UV Damage.

Snow JA, Murthy V, Dacus D, Hu C, Wallace NA
Pathogens (Basel, Switzerland) 2019 Nov 26;8(4)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Phospho-Cdc25A (Thr507) in HeLa cells. Samples were incubated with Phospho-Cdc25A (Thr507) polyclonal antibody (Product # PA5-12564) using a dilution of 1:25 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG at a dilution of 1:400 (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Phospho-Cdc25A (Thr507) in formalin-fixed and paraffin-embedded human cancer tissue. Samples were incubated with Phospho-Cdc25A (Thr507) polyclonal antibody (Product # PA5-12564) which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 beta-HPV 8E6 attenuates CHK1 phosphorylation. ( A ) Representative immunoblots of untreated hTERT HFKs with vector control (LXSN) and beta-HPV 8E6 cell lines. Nucleolin was used as a loading control. ( B ) mRNA expression level of CHEK1 in vector control (LXSN) and beta-HPV 8E6 expressing primary HFKs as measured by RT-qPCR and normalized towards the expression level of beta-actin. Data shown in figures are the means of +-SE of three independent experiments. ( C ) Representative immunoblots of hTERT HFKs with vector control (LXSN) and beta-HPV 8E6 harvested 0-6 h post 5mJ/cm 2 UVR. Nucleolin was used as a loading control. ( D ) Representative immunoblots of primary HFKs with vector control (LXSN) and beta-HPV 8E6 harvested 0-8 h post 5 mJ/cm 2 UVR. Nucleolin was used as a loading control. ( E ) Representative immunoblots of hTERT HFKs with vector control (LXSN) and beta-HPV 8E6 harvested 0-8 h post 5 mJ/cm 2 UVR. Nucleolin was used as a loading control. ( A, C - E ) The numbers above bands represent quantification by densitometry. This is shown relative to untreated cells within the same cell line and normalized to the loading control. ( F ) Cell cycle analysis of hTERT HFKs with LXSN vector control and beta-HPV 8E6 1 h post 5 mJ/cm 2 UVR.