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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [5]
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Validation data
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- Product number
- LS-C337450 - Provider product page
- Provider
- LSBio
- Product name
- SETD7 / SET7 Antibody (clone OTI2D10) LS-C337450
- Antibody type
- Monoclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse, Rat, Canine, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI2D10
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SETD7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SETD7.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of extracts (35ug) from 9 different cell lines by using anti-SETD7 monoclonal antibody.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of extracts (10ug) from 9 Human tissue by using anti-SETD7 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SETD7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-SETD7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SETD7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-SETD7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-SETD7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
