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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
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- Product number
- MAB1053 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB1053, RRID:AB_1580528
- Product name
- SETD7 monoclonal antibody, clone s4E5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against full length recombinant SETD7.
- Isotype
- IgG
- Antibody clone number
- s4E5
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Protein demethylation required for DNA methylation.
Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9-AdoMet.
Structure and catalytic mechanism of the human histone methyltransferase SET7/9.
Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation.
Hotz HR, Peters AH
Nature genetics 2009 Jan;41(1):10-1
Nature genetics 2009 Jan;41(1):10-1
Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9-AdoMet.
Kwon T, Chang JH, Kwak E, Lee CW, Joachimiak A, Kim YC, Lee J, Cho Y
The EMBO journal 2003 Jan 15;22(2):292-303
The EMBO journal 2003 Jan 15;22(2):292-303
Structure and catalytic mechanism of the human histone methyltransferase SET7/9.
Xiao B, Jing C, Wilson JR, Walker PA, Vasisht N, Kelly G, Howell S, Taylor IA, Blackburn GM, Gamblin SJ
Nature 2003 Feb 6;421(6923):652-6
Nature 2003 Feb 6;421(6923):652-6
Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation.
Nishioka K, Chuikov S, Sarma K, Erdjument-Bromage H, Allis CD, Tempst P, Reinberg D
Genes & development 2002 Feb 15;16(4):479-89
Genes & development 2002 Feb 15;16(4):479-89
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- Experimental details
- Immunoblot analysis of recombinant SETD7 was resolved by electrophoresis , transferred to PVDF membrane and probed with SETD7 monoclonal antibody , clone s4E5 (1 : 1000) (Cat # MAB1053) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicated SETD7 (~ 50 kDa) .
