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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB2369 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2369, RRID:AB_1580532
- Product name
- SETD7 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of SETD7.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9-AdoMet.
Structure and catalytic mechanism of the human histone methyltransferase SET7/9.
Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1.
Kwon T, Chang JH, Kwak E, Lee CW, Joachimiak A, Kim YC, Lee J, Cho Y
The EMBO journal 2003 Jan 15;22(2):292-303
The EMBO journal 2003 Jan 15;22(2):292-303
Structure and catalytic mechanism of the human histone methyltransferase SET7/9.
Xiao B, Jing C, Wilson JR, Walker PA, Vasisht N, Kelly G, Howell S, Taylor IA, Blackburn GM, Gamblin SJ
Nature 2003 Feb 6;421(6923):652-6
Nature 2003 Feb 6;421(6923):652-6
Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1.
Wysocka J, Myers MP, Laherty CD, Eisenman RN, Herr W
Genes & development 2003 Apr 1;17(7):896-911
Genes & development 2003 Apr 1;17(7):896-911
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The SETD7 polyclonal antibody (Cat # PAB2369) is used in Western blot to detect SET9 in mouse brain tissue lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SETD7 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the SETD7 polyclonal antibody (Cat # PAB2369) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
