Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-76734 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FBP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FBP1. Samples were incubated with FBP1 polyclonal antibody (Product # PA5-76734).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-FBP1 Polyclonal Antibody (Product # PA5-76734) and 36 kDa band corresponding to FBP1 was observed in MCF7, HT-29 and little or no expression was observed in SK-BR-3, T-47D, MDA-MB-231, HCT 116 and Hep G2 which are reported negative for FBP1 expression. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), SK-BR-3 (Lane 2), T-47D (Lane 3), MDA-MB-231 (Lane 4), HT-29 (Lane 5), HCT 116 (Lane 6) and Hep G2 (Lane 7), were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of FBP1 in MCF7 cells. Samples were incubated with FBP1 polyclonal antibody (Product # PA5-76734).