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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- MA5-31704 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CLGN Monoclonal Antibody (9C8G10)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-31704 has been tested in indirect ELISA.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 9C8G10
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CLGN in LNCaP (1), HepG2 (2), PC-3 (3), and Raji (4) cell lysate. Samples were incubated with CLGN monoclonal antibody (Product # MA5-31704) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CLGN in human CLGN recombinant protein. Sample was incubated with CLGN monoclonal antibody (Product # MA5-31704) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CLGN in HEK293 (1) and CLGN cell lysate. Samples were incubated with CLGN monoclonal antibody (Product # MA5-31704) using a dilution of 1:500-1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA analysis of CLGN in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with CLGN monoclonal antibody (Product # MA5-31704) using a dilution of 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of CLGN in HepG2 cells (green). Sample was incubated with CLGN monoclonal antibody (Product # MA5-31704) using a dilution of 1:75 followed by DRAQ5 fluorescent DNA dye (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CLGN in paraffin-embedded breast cancer tissue. Sample was incubated with CLGN monoclonal antibody (Product # MA5-31704) using a dilution of 1:200-1:1000 followed by DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CLGN in paraffin-embedded esophageal cancer tissue. Sample was incubated with CLGN monoclonal antibody (Product # MA5-31704) using a dilution of 1:200-1:1000 followed by DAB staining.
