PA5-76632

antibody from Invitrogen Antibodies

Targeting: NR0B2 SHP

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-76632

Provider

Invitrogen Antibodies

Product name

NR0B2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Gypenosides ameliorate high-fat diet-induced non-alcoholic steatohepatitis via farnesoid X receptor activation.

Li H, Xi Y, Liu H, Xin X
Frontiers in nutrition 2022;9:914079

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 6 Effects of Gyps on hepatic SHP mRNA and protein levels in WT and FXR KO mice. (A1) Quantification of hepatic SHP protein levels in different groups of WT mice. (A2) Quantification of hepatic SHP protein levels in different groups in FXR KO mice. (B1) Relative expression levels of SHP mRNA in different groups in WT and FXR KO mice. (B2) Quantification of hepatic SHP protein levels in the ND and FXR KO ND groups. (C1) WB analysis of hepatic SHP protein expression in different groups of WT mice. (C2) WB analysis of hepatic SHP protein expression in different groups of FXR KO mice. (C3) WB analysis of hepatic SHP protein expression in ND and FXR KO ND groups. FXR, farnesoid X receptor; SHP, small heterodimer partner; ND, normal diet; HFD, high-fat diet; Gyps, gypenosides; WT, wild-type; KO, knockout; WB, western blot. * p < 0.05, ** p < 0.01.