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- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MA5-17064 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP3A4 Monoclonal Antibody (3H8)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17064 targets CYP3A4 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples. The MA5-17064 immunogen is purified recombinant fragment of human CYP3A4 expressed in E. Coli. MA5-17064 detects CYP3A4 which has a predicted molecular weight of approximately 57.3kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3H8
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Commiphora myrrha (Nees) Engl. resin extracts induce phase-I cytochrome P450 2C8, 2C9, 2C19, and 3A4 isoenzyme expressions in human hepatocellular carcinoma (HepG2) cells.
Overcoming bioprocess bottlenecks in the large-scale expansion of high-quality hiPSC aggregates in vertical-wheel stirred suspension bioreactors.
Stirred suspension bioreactors maintain naïve pluripotency of human pluripotent stem cells.
iTAG-RNA Isolates Cell-Specific Transcriptional Responses to Environmental Stimuli and Identifies an RNA-Based Endocrine Axis.
Improving Drug Discovery by Nucleic Acid Delivery in Engineered Human Microlivers.
An in vitro system for measuring genotoxicity mediated by human CYP3A4 in Saccharomyces cerevisiae.
Alehaideb Z, Alatar G, Nehdi A, Albaz A, Al-Eidi H, Almutairi M, Hawsa E, Alshuail N, Matou-Nasri S
Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society 2021 May;29(5):361-368
Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society 2021 May;29(5):361-368
Overcoming bioprocess bottlenecks in the large-scale expansion of high-quality hiPSC aggregates in vertical-wheel stirred suspension bioreactors.
Borys BS, Dang T, So T, Rohani L, Revay T, Walsh T, Thompson M, Argiropoulos B, Rancourt DE, Jung S, Hashimura Y, Lee B, Kallos MS
Stem cell research & therapy 2021 Jan 13;12(1):55
Stem cell research & therapy 2021 Jan 13;12(1):55
Stirred suspension bioreactors maintain naïve pluripotency of human pluripotent stem cells.
Rohani L, Borys BS, Razian G, Naghsh P, Liu S, Johnson AA, Machiraju P, Holland H, Lewis IA, Groves RA, Toms D, Gordon PMK, Li JW, So T, Dang T, Kallos MS, Rancourt DE
Communications biology 2020 Sep 7;3(1):492
Communications biology 2020 Sep 7;3(1):492
iTAG-RNA Isolates Cell-Specific Transcriptional Responses to Environmental Stimuli and Identifies an RNA-Based Endocrine Axis.
Darr J, Tomar A, Lassi M, Gerlini R, Berti L, Hering A, Scheid F, Hrabě de Angelis M, Witting M, Teperino R
Cell reports 2020 Mar 3;30(9):3183-3194.e4
Cell reports 2020 Mar 3;30(9):3183-3194.e4
Improving Drug Discovery by Nucleic Acid Delivery in Engineered Human Microlivers.
Mancio-Silva L, Fleming HE, Miller AB, Milstein S, Liebow A, Haslett P, Sepp-Lorenzino L, Bhatia SN
Cell metabolism 2019 Mar 5;29(3):727-735.e3
Cell metabolism 2019 Mar 5;29(3):727-735.e3
An in vitro system for measuring genotoxicity mediated by human CYP3A4 in Saccharomyces cerevisiae.
Fasullo M, Freedland J, St John N, Cera C, Egner P, Hartog M, Ding X
Environmental and molecular mutagenesis 2017 May;58(4):217-227
Environmental and molecular mutagenesis 2017 May;58(4):217-227
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HepG2 cells using CYP3A4 monoclonal antibody (Product # MA5-17064) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using CYP3A4 monoclonal antibody (Product # MA5-17064) (green) and negative control (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Engineering Liver Metabolism in MPCCs (A) CYP3A4 mRNA levels at day 3 post-transfection. Values (mean +- SEM, n = 2) were normalized to untreated MPCCs. p < 0.001. (B) Representative images of hepatocyte islands in MPCCs probed with CYP3A4 antibodies (green) on day 4 post-transfection. Hepatocyte and fibroblast nuclei are shown in blue. The white dotted line delineates the island border of hepatocytes. Scale bar, 50 mum. (C and D) Schematic reaction of the luminescence-based CYP3A4 activity assay (C) and relative CYP3A4 activity measured in MPCCs (D). Values were normalized to the corresponding untreated cells. Presented data are mean +- SEM (n = 3). p < 0.0001. (E) Relative human albumin levels secreted in MPCCs, as determined by ELISA. Values (mean +- SEM, n = 2) were normalized to the corresponding untreated condition. See Figure S1 A for urea levels. (F) CYP3A4 and CYP2D6 mRNA levels at day 3 post-transfection with CYP3A4 and CYP2D6 siRNA duplexes, alone or in combination. Graph bars represent mean +- SEM (n = 3). p < 0.01. (G) Relative CYP3A4 activity in the same conditions as (F), using proluciferin. Graph bars represent mean +- SEM (n = 2). **p < 0.01. *p < 0.05. See Figure S1 B for CYP3A4 activity measured with testosterone substrate.
