PA5-14896

antibody from Invitrogen Antibodies

Targeting: CYP3A4 CYP3A3

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-14896 - Provider product page
Provider: Invitrogen Antibodies
Product name: CYP3A4 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 200 µL
Concentration: 0.5 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

CYP3A4 protein structure - PA5-14896 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Effect of beta-NF and EtOH treatment on cytochrome P450 (CYP) isoforms' expression in undifferentiated SH-SY5Y cells. ( a - d ) The graphs show the relative protein levels quantification by Western blot for the CYP2D6 ( a ), 2E1 ( b ), 1A1 ( c ), and 3A4 ( d ) isoforms after treating cells with the inducers for 48 h (see Section 4 ). Data was acquired by measuring the fluorescent intensity per pixel on each band. The relative amount of protein normalised with beta-actin as a housekeeping protein for each condition was plotted as fold-increase and compared to the control, which was given a value of 1. Columns represent the mean +- SEM of at least three different experiments. Statistical significance was analyzed by one-way ANOVA followed by a Tukey post-test (* p < 0.05; ** p < 0.01). ( e ) Top panel shows a representative blot of beta-actin housekeeping protein detected with secondary antibody Cy3 (green) in control (2nd lane), beta-NF (3rd lane) and EtOH (4th lane) treatments. ( e ) Bottom panel shows representative blots of each isoform detected with secondary antibody Cy5 (red) in the mentioned conditions.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Effect of beta-NF and EtOH treatment on cytochrome P450 (CYP) isoforms' expression in undifferentiated SH-SY5Y cells. ( a - d ) The graphs show the relative protein levels quantification by Western blot for the CYP2D6 ( a ), 2E1 ( b ), 1A1 ( c ), and 3A4 ( d ) isoforms after treating cells with the inducers for 48 h (see Section 4 ). Data was acquired by measuring the fluorescent intensity per pixel on each band. The relative amount of protein normalised with beta-actin as a housekeeping protein for each condition was plotted as fold-increase and compared to the control, which was given a value of 1. Columns represent the mean +- SEM of at least three different experiments. Statistical significance was analyzed by one-way ANOVA followed by a Tukey post-test (* p < 0.05; ** p < 0.01). ( e ) Top panel shows a representative blot of beta-actin housekeeping protein detected with secondary antibody Cy3 (green) in control (2nd lane), beta-NF (3rd lane) and EtOH (4th lane) treatments. ( e ) Bottom panel shows representative blots of each isoform detected with secondary antibody Cy5 (red) in the mentioned conditions.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 7 Immunostaining of CYP3A4 in SH-SY5Y cells after beta-NF and EtOH treatment. Representative immunofluorescence images of each experimental condition. Column 1 represents mitochondrial stain (red), column 2 represents ER-Green Fluorescent Protein (GFP) stain (green), and column 3 reports the CYP3A4 staining (blue). Column 4 represents the merge between CYP3A4 and mitochondria channel, column 5 represents the merge between CYP3A4 and ER-GFP, and column 6 represents the merge of the three channels. R Values: Pearson's correlation coefficient. Scale bar: 20 um.