F55084-0.2ML

antibody from NSJ Bioreagents

Targeting: CYP3A4 CYP3A3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F55084-0.2ML

Provider

NSJ Bioreagents

Product name

CYP3A4 Antibody / Cytochrome P450 3A4

Antibody type

Polyclonal

Description

Purified

Reactivity

Human

Host

Rabbit

Vial size

0.2 ml, 0.05 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the CYP3A4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HepG2, 2) A549, 3) HT-29 and 4) OVCAR3 cell lysate with CYP3A4 antibody. Expected molecular weight: 50-57 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human liver tissue with CYP3A4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human CEM cells with CYP3A4 antibody; Blue=isotype control, Green= CYP3A4 antibody.