Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF356 - Provider product page
- Provider
- R&D Systems
- Product name
- Human HVEM/TNFRSF14 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human HVEM/TNFRSF14 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse HVEM/TNFRSF14 and recombinant rat HVEM/TNFRSF14 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q92956- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references CD160 isoforms and regulation of CD4 and CD8 T-cell responses.
Search for polymorphisms in the genes for herpesvirus entry mediator, nectin-1, and nectin-2 in immune seronegative individuals.
El-Far M, Pellerin C, Pilote L, Fortin JF, Lessard IA, Peretz Y, Wardrop E, Salois P, Bethell RC, Cordingley MG, Kukolj G
Journal of translational medicine 2014 Sep 2;12:217
Journal of translational medicine 2014 Sep 2;12:217
Search for polymorphisms in the genes for herpesvirus entry mediator, nectin-1, and nectin-2 in immune seronegative individuals.
Struyf F, Posavad CM, Keyaerts E, Van Ranst M, Corey L, Spear PG
The Journal of infectious diseases 2002 Jan 1;185(1):36-44
The Journal of infectious diseases 2002 Jan 1;185(1):36-44
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human HVEM/TNFRSF14 by Western Blot. Western blot shows lysates of human thymus tissue and human peripheral blood mononuclear cells (PBMCs). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HVEM/TNFRSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF356) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HVEM/TNFRSF14 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- HVEM/TNFRSF14 in Human Spleen. HVEM/TNFRSF14 was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human HVEM/TNFRSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF356) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
