PA5-116514

antibody from Invitrogen Antibodies

Targeting: PCBP1 hnRNP-E1, hnRNP-X, HNRPE1, HNRPX

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA5-116514

Provider

Invitrogen Antibodies

Product name

PCBP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Samples: NIH/3T3, HepG2, MCF7, Mouse liver, Rat liver Immunogen sequence: LRLVVPATQC GSLIGKGGCK IKEIRESTGA QVQVAGDMLP NSTERAITIA GVPQSVTECV KQICLVMLET LSQSPQGRVM TIPYQPMPAS SPVICAGGQD R

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.01 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PCBP1 using PCBP1 Polyclonal Antibody (Product # PA5-116514) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of PCBP1 in NIH-3T3 cells using PCBP1 Polyclonal Antibody (Product # PA5-116514) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of PCBP1 in C6 cells using PCBP1 Polyclonal Antibody (Product # PA5-116514) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of PCBP1 in NIH-3T3 cells using PCBP1 Polyclonal Antibody (Product # PA5-116514) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of PCBP1 in U-2 OS cells using PCBP1 Polyclonal Antibody (Product # PA5-116514) at a dilution of 1:100. Blue: DAPI for nuclear staining.