14-9200-80

antibody from Invitrogen Antibodies

Targeting: CD200 MOX1, MOX2, MRC, OX-2

Provider product page for 14-9200-80

Immunohistochemistry

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [6]
Comments [0]
Validations
Flow cytometry [1]
Other assay [5]

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Product number: 14-9200-80 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD200 Monoclonal Antibody (OX104), eBioscience™
Antibody type: Monoclonal
Antigen: Other
Description: Description: The monoclonal antibody OX104 recognizes human CD200 also known as OX2. CD200 is a member of the Ig superfamily with 2 Ig domains, a transmembrane and cytoplasmic domain. CD200 is expressed on resting and activated B cells, a subset of resting and activated T cells, keratinocytes, peripheral and central nerve cells, follicular dendritic cells and ovarian cells. The interaction with CD200R results in macrophage activation (IL-6 production), inhibition of mast cell degranulation along with reduced TNFalpha and IL-13 secretion and overall attenuation of the activation status of lymphocytes. A role has also been suggested in maternal tolerance as expression of CD200 is also present on the trophoblast. Applications Reported: This OX104 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections. Applications Tested: This OX104 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: OX104
Vial size: 25 µg
Concentration: 0.5 mg/mL
Storage: 4° C

CD200 protein structure - 14-9200-80 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 1 Recombinant human OX2CD4d3+4 binds rat OX2 receptor (OX2R). Flow cytometry analysis shows that human OX2-coated beads bound rat resident peritoneal cells (RPCs) coated in OX45 (anti-rat CD48) monoclonal antibody (mAb) (thick line), but that binding was blocked back to a negative control (CD4d3+4-coated beads, thin line) by OX102 (anti-rat OX2R) mAb (broken line).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 3 OX2 is expressed on thymocytes and at increased levels on single positive, lineage-committed thymocytes. (a) Flow cytometry shows that almost all thymocytes were stained with the OX104 monoclonal antibody (mAb) (thick line) above an isotype-matched negative control, OX1 (thin line). (b) Thymocytes were gated, according to CD4 and CD8 expression, into: (R1) CD4 + CD8 + double-positives (DPs); (R2) CD8 + single-positives (SPs); and (R3) CD4 + SPs. The level of OX2 expression was plotted as a function of the normalized cell number from each region in (b) with R3 CD4 + SPs (bold line), R2 CD8 + SPs (dotted line) and R1 CD4 + CD8 + DPs (thin line) (c).