MA5-32615

antibody from Invitrogen Antibodies

Targeting: TIA1 TIA-1

Western blot

Immunoprecipitation

Antibody data

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Validation data

Product number: MA5-32615 - Provider product page
Provider: Invitrogen Antibodies
Product name: TIA-1 Recombinant Rabbit Monoclonal Antibody (JM42-11)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Antibody clone number: JM42-11
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

TIA1 protein structure - MA5-32615 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemical analysis of TIA-1 in 293T cells using a TIA-1 Monoclonal antibody (Product # MA5-32615) as seen in red. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemical analysis of TIA-1 in A431 cells using a TIA-1 Monoclonal antibody (Product # MA5-32615) as seen in red. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemical analysis of TIA-1 in Hela cells using a TIA-1 Monoclonal antibody (Product # MA5-32615) as seen in red. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of TIA-1 with Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS (10 min, room temp) and blocked with 1% Blocker BSA (15 min, room temp), incubated with recombinant monoclonal TIA-1 (Product # MA5-32615) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS, followed by Alexa Fluor 594 Goat anti-Rabbit IgG at 1:1,000 dilution. DAPI was used to stain the cell nuclei (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of TIA-1 of paraffin-embedded Human tonsil tissue using a TIA-1 Monoclonal antibody (Product #MA5-32615). Counter stained with hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of TIA-1 of paraffin-embedded Human spleen tissue using a TIA-1 Monoclonal antibody (Product #MA5-32615). Counter stained with hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of paraffin-embedded TIA-1 in human tonsil tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS. Incubation was done with monoclonal TIA-1 antibody (Product # MA5-32615) at a dilution of 1:200 (1h at RT). The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of paraffin-embedded TIA-1 in human spleen tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS. Incubation was done with monoclonal TIA-1 antibody (Product # MA5-32615) at a dilution of 1:1,000 (1h at RT). The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.