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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- V8438 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- TIA1 Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific TIA1 antibody is suitable for use in Immunofluorescence/Western blot applications with human samples.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- TIA1/1313
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the TIA1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human A) HepG2, B) PC-3 and C) HeLa cell lysate wiht TIA-1 antibody (clone TIA1/1313). The long and short forms differ by 17 amino acids. Predicted molecular weight: ~43 kDa and ~15 kDa (granulocyte-associated isoform).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PFA-fixed human HeLa cells with TIA1 antibody (green, clone TIA1/1313) and Reddot nuclear stain (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free TIA1 antibody (clone TIA1/1313) as confirmation of integrity and purity.
