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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-29957 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ACAA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2.
- Concentration
- 0.31 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ACAA1 Polyclonal Antibody (Product # PA5-29957). Sample (30 µg of whole cell lysate). Lane A: Hep G2. 10% SDS PAGE. ACAA1 Polyclonal Antibody . ACAA1 Polyclonal Antibody (Product # PA5-29957) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ACAA1 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: ACAA1 Polyclonal Antibody (Product # PA5-29957) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ACAA1 Polyclonal Antibody detects ACAA1 protein at Peroxisome by immunofluorescent analysis. Sample: HepG2 cells were fixed in -20ºC 100% MeOH for 5 min. Green: ACAA1 protein stained by ACAA1 Polyclonal Antibody (Product # PA5-29957) diluted at 1:500. Blue: Hoechst 33343 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ACAA1 was performed in paraffin-embedded mouse liver tissue using ACAA1 Polyclonal Antibody (Product # PA5-29957) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
