TA506118

antibody from OriGene

Targeting: ZSCAN21 DKFZp434L134, NY-REN-21, Zipro1, ZNF38

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA506118

Provider

OriGene

Proper citation

OriGene Cat#TA506118, RRID:AB_2623616

Product name

ZSCAN21 mouse monoclonal antibody,clone OTI2E5

Antibody type

Monoclonal

Description

ZSCAN21 mouse monoclonal antibody,clone OTI2E5

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

ZSCAN21

Isotype

IgG

Antibody clone number

OTI2E5

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ZSCAN21 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZSCAN21 (RC208411, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZSCAN21.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-ZSCAN21 mouse monoclonal antibody (TA506118) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ZSCAN21(RC208411).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ZSCAN21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506118)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ZSCAN21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506118)

Validation comment

IHC