PA5-103785

antibody from Invitrogen Antibodies

Targeting: DGAT2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-103785

Provider

Invitrogen Antibodies

Product name

DGAT2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of total DGAT2.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

Accumulation of cholesterol, triglycerides and ceramides in hepatocellular carcinomas of diethylnitrosamine injected mice.

Haberl EM, Pohl R, Rein-Fischboeck L, Höring M, Krautbauer S, Liebisch G, Buechler C
Lipids in health and disease 2021 Oct 10;20(1):135

---

Ferroptotic cell death triggered by conjugated linolenic acids is mediated by ACSL1.

Beatty A, Singh T, Tyurina YY, Tyurin VA, Samovich S, Nicolas E, Maslar K, Zhou Y, Cai KQ, Tan Y, Doll S, Conrad M, Subramanian A, Bayır H, Kagan VE, Rennefahrt U, Peterson JR
Nature communications 2021 Apr 14;12(1):2244

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of DGAT2 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and DGAT2 polyclonal antibody (Product # PA5-103785) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 Enzymes with a role in TG synthesis in the normal tissues (NT) and tumor tissues (TT) of mice injected with diethylnitrosamine. a Expression of ACC, pACC, FAS, and HSL. b Expression of DGAT1, DGAT2, pAMPK, AMPK and CD36. c Expression of LPL, PGC1alpha and LIPC. Cyclophilin A (CycA) was a further housekeeping protein analysed. d Ratio of pACC/ACC and pAMPK/AMPK in NT and TT. e Quantification of LPL protein. f Quantification of PGC1alpha protein. g Quantification of LIPC protein. h Quantification of proteins not changed in the TT. ( n = 6-7). * P < 0.05; *** P < 0.001