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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- ASR-037-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 5HT7 Receptor/HTR7 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1, 2. Anti-5HT7 Receptor/HTR7 (extracellular) Antibody (#ASR-037), (1:400). 3, 4. Anti-5HT7 Receptor/HTR7 (extracellular) Antibody , preincubated with 5HT7 Receptor/HTR7 (extracellular) Blocking Peptide (#BLP-SR037).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1, 2. Anti-5HT7 Receptor/HTR7 (extracellular) Antibody (#ASR-037), (1:400). 3, 4. Anti-5HT7 Receptor/HTR7 (extracellular) Antibody , preincubated with 5HT7 Receptor/HTR7 (extracellular) Blocking Peptide (#BLP-SR037).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse BV-2 microglia cell line lysate (lanes 1 and 4), human THP-1 monocytic leukemia cell line lysate (lanes 2 and 5) and human MEG-01 megakaryoblastic leukemiacell line lysate (lanes 3 and 6): - 1-3. Anti-5HT7 Receptor/HTR7 (extracellular) Antibody (#ASR-037), (1:400). 4-6. Anti-5HT7 Receptor/HTR7 (extracellular) Antibody , preincubated with 5HT7 Receptor/HTR7 (extracellular) Blocking Peptide (#BLP-SR037).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of5HT7 Receptor in rat dorsal raphe nucleus (DRN) - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-5HT7 Receptor/HTR7 (extracellular) Antibody (#ASR-037), (1:300), followed by goat Anti-rabbit-AlexaFluor-488. A.5HT7Rimmunoreactivity (green) appears in neuronal profiles in the lateral DRN (arrows). B. Pre-incubation of the Antibody with 5HT7 Receptor/HTR7 (extracellular) Blocking Peptide (#BLP-SR037), suppresses staining. Cell nuclei are stained with DAPI (blue). LDRN = lateral DRN, MDRN = medial DRN.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of5HT7 Receptor in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-5HT7 Receptor/HTR7 (extracellular) Antibody (#ASR-037), (1:300), followed by goat Anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal dentate gyrus region, shows5HT7Rimmunoreactivity (green) in neuronal profiles in the hilus (vertical arrows) and in glial profiles in the outer molecular layer (OML, horizontal arrows). B. Pre-incubation of the Antibody with 5HT7 Receptor/HTR7 (extracellular) Blocking Peptide (#BLP-SR037), suppressed staining. Cell nuclei are stained with DAPI (blue). H = hilus, G = granule layer, OML= outer molecular layer.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of 5HT7 Receptor in live intact human MEG-01 megakaryoblastic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-5HT7 Receptor/HTR7 (extracellular) Antibody (#ASR-037), (5 µg) + goat- Anti-rabbit-FITC.
