GTX632189

ME1 antibody from GeneTex

Validated within the Antibodypedia Validation Initative

Western blot

Enhanced validation

Immunocytochemistry

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for GTX632189

Antibody Data

Product number

GTX632189

Provider

GeneTex

Product name

ME1 antibody [GT979]

Provider product page

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Mouse

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ME1 antibody detects ME1 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody (GTX632189) diluted at a dilution of 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody [GT979] (GTX632189) diluted at 1:1000.

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody [GT979] (GTX632189) diluted at 1:1000.

KD Reagent Type

siRNA

Downregulation

>50%

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod no

Proprietary.

Antibody Lot Number

42037

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Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ME1 antibody [GT979] detects ME1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ME1 protein stained by ME1 antibody [GT979] (GTX632189) diluted at 1:400.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ME1 antibody [GT979] detects ME1 protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. ME1 antibody [GT979] (GTX632189) diluted at 1:250.