H00004199-M03

antibody from Abnova Corporation

Targeting: ME1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004199-M03

Provider

Abnova Corporation

Proper citation

Product name

ME1 monoclonal antibody (M03), clone 3H5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant ME1.

Antigen sequence

MEPEAPRRRHTHQRGYLLTRNPHLNKDLAFTLEER
QQLNIHGLLPPSFNSQEIQVLRVVKNFEHLNSDFD
RYLLLMDLQDRNEKLFYRVLTSDIEKFMPIVYTPT
VGLACQQYSLVFRKPRGLFITIHDRGHIASVLNAW
PEDVIKAIVVTDGERILGLGDLGCNGMGIPVGKLA
LYTACGGMNPQECLPVILDVGTENEELLKDPLYIG
LRQRRVRGSEYDDFLDEFMEAVSSKYGMNCLIQFE
DFANVNAFRLLNKYRNQYCTFNDDIQGTASVAVAG
LLAALRITKNKLSDQTILFQGAGEAALGIAHLIVM
ALEKEGLPKEKAIKKIWLVDSKGLIVKGRASLTQE
KEKFAHEHEEMKNLEAIVQEIKPTALIGVAAIGGA
FSEQILKDMAAFNERPIIFALSNPTSKAECSAEQC
YKITKGRAIFASGSPFDPVTLPNGQTLYPGQGNNS
YVFPGVALGVVACGLRQITDNIFLTTAEVIAQQVS
DKHLEEGRLYPPLNTIRDVSLKIAEKIVKDAYQEK
TATVYPEPQNKEAFVRSQMYSTDYDQILPDCYSWP
EEVQKIQTKVDQ


Isotype

IgG

Antibody clone number

3H5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Role for malic enzyme, pyruvate carboxylation, and mitochondrial malate import in glucose-stimulated insulin secretion.

Heart E, Cline GW, Collis LP, Pongratz RL, Gray JP, Smith PJ
American journal of physiology. Endocrinology and metabolism 2009 Jun;296(6):E1354-62

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

ME1 monoclonal antibody (M03), clone 3H5. Western Blot analysis of ME1 expression in HepG2 ( Cat # L019V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ME1 expression in transfected 293T cell line by ME1 monoclonal antibody (M03), clone 3H5.Lane 1: ME1 transfected lysate(64.1 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ME1 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to ME1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.5 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol