Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [6]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-32398 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-32398, RRID:AB_2143825
- Product name
- Rabbit Polyclonal ME1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Sex-dimorphic estrogen receptor regulation of ventromedial hypothalamic nucleus glucoregulatory neuron adrenergic receptor expression in hypoglycemic male and female rats.
Uddin MM, Mahmood ASMH, Ibrahim MMH, Briski KP
Brain research 2019 Oct 1;1720:146311
Brain research 2019 Oct 1;1720:146311
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ME1 Antibody [NBP1-32398] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ME1 Antibody [NBP1-32398] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ME1 Antibody [NBP1-32398] - Immunoprecipitation of ME1 protein from HeLa whole cell extracts using 5 ug of ME1 antibody. Western blot analysis was performed using ME1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ME1 Antibody [NBP1-32398] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ME1 Antibody [NBP1-32398] - Sample (30 ug of whole cell lysate) A: PC-12 7. 5% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ME1 Antibody [NBP1-32398] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ME1 Antibody [NBP1-32398] - Paraffin-embedded human ovarian carcinoma. ME1 antibody dilution: 1:500.