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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-21550 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ME1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, JC, BCL-1, PC-12.
- Concentration
- 0.94 mg/mL
Submitted references Loss of glucose 6-phosphate dehydrogenase function increases oxidative stress and glutaminolysis in metastasizing melanoma cells.
Aurora AB, Khivansara V, Leach A, Gill JG, Martin-Sandoval M, Yang C, Kasitinon SY, Bezwada D, Tasdogan A, Gu W, Mathews TP, Zhao Z, DeBerardinis RJ, Morrison SJ
Proceedings of the National Academy of Sciences of the United States of America 2022 Feb 8;119(6)
Proceedings of the National Academy of Sciences of the United States of America 2022 Feb 8;119(6)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ME1 using Various whole cell extracts (30 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a ME1 polyclonal antibody (Product # PA5-21550) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ME1 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ME1 Polyclonal Antibody (Product # PA5-21550) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ME1 Polyclonal Antibody (Product # PA5-21550). Sample (30 µg of whole cell lysate). Lane A: PC-12. 7.5% SDS PAGE. ME1 Polyclonal Antibody (Product # PA5-21550) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ME1 Polyclonal Antibody (Product # PA5-21550). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: JC. Lane C: BCL-1. 7.5% SDS PAGE. ME1 Polyclonal Antibody (Product # PA5-21550) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ME1 in paraformaldehyde-fixed HeLa cells using a ME1 polyclonal antibody (Product # PA5-21550) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ME1 antibody (Product # PA5-21550) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ME1 Polyclonal Antibody detects ME1 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. ME1 Polyclonal Antibody (Product # PA5-21550) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of ME1 was performed in HeLa whole cell extracts using 5 µg of ME1 Polyclonal Antibody (Product # PA5-21550). Samples were transferred to a membrane and probed with ME1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
