H00007039-D01P

antibody from Abnova Corporation

Targeting: TGFA

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007039-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007039-D01P, RRID:AB_1715656

Product name

TGFA purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human TGFA protein.

Antigen sequence

MVPSAGQLALFALGIVLAACQALENSTSPLSADPP
VAAAVVSHFNDCPDSHTQFCFHGTCRFLVQEDKPA
CVCHSGYVGARCEHADLLAVVAASQKKQAITALVV
VSIVALAVLIITCVLIHCCQVRKHCEWCRALICRH
EKPSALLKGRTACCHSETVV


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of TGFA expression in transfected 293T cell line (H00007039-T01) by TGFA MaxPab polyclonal antibody.Lane 1: TGFA transfected lysate(17.00 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between TGFA and ADAM17. HeLa cells were stained with anti-TGFA rabbit purified polyclonal 1:1200 and anti-ADAM17 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)