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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- ELISA [1]
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- Product number
- 500-P16-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TGF alpha Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TGF-alpha. Anti-Human TGF-alpha-specific antibody was purified by affinity chromatography employing an immobilized Human TGF-alpha matrix. Sandwich ELISA: To detect Human TGF-alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TGF-alpha (500-P16Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TGF-alpha. Western Blot: To detect Human TGF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human TGF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Skin-derived micro-organs induce angiogenesis in rabbits.
Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
Regulation of daily locomotor activity and sleep by hypothalamic EGF receptor signaling.
Specificity of the localization of transforming growth factor-alpha immunoreactivity in colon mucosa.
Hasson E, Gallula J, Shimoni Y, Grad-Itach E, Marikovsky M, Mitrani E
Journal of vascular research 2006;43(2):139-48
Journal of vascular research 2006;43(2):139-48
Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
Li Y, Fan J, Chen M, Li W, Woodley DT
The Journal of investigative dermatology 2006 Sep;126(9):2096-105
The Journal of investigative dermatology 2006 Sep;126(9):2096-105
Regulation of daily locomotor activity and sleep by hypothalamic EGF receptor signaling.
Kramer A, Yang FC, Snodgrass P, Li X, Scammell TE, Davis FC, Weitz CJ
Science (New York, N.Y.) 2001 Dec 21;294(5551):2511-5
Science (New York, N.Y.) 2001 Dec 21;294(5551):2511-5
Specificity of the localization of transforming growth factor-alpha immunoreactivity in colon mucosa.
Xian CJ, Mardell CE, Read LC
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1999 Jul;47(7):949-58
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1999 Jul;47(7):949-58
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- Invitrogen Antibodies (provider)
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- Experimental details
- Sandwich ELISA: To detect Human TGF-alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of TGF alpha Polyclonal Antibody (Product # 500-P16-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech TGF alpha Polyclonal Antibody, Biotin (Product # 500-P16BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TGF-alpha.
