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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB-239-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human TGF-alpha Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human TGF-alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) AR, rhBTC, rhEGF, rhHB-EGF, rhHRG-alpha , and rhHRG-beta is observed. Neutralizes the biological activity of rhTGF-alpha , but will not neutralize the biological activity of rhEGF.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P01135- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by TGF-alpha and Neutralization by Human TGF-alpha Antibody. Recombinant Human TGF-alpha (Catalog # 239-A) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human TGF-alpha (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TGF-alpha Polyclonal Antibody (Catalog # AB-239-NA). The ND50 is typically 0.4-0.8 µg/mL.
